Glutamyl Transpeptidase Optimization II-PR-A

Allium sativum

Garlic

Tier IIGlutamyl Transpeptidase Optimization

Target Trait

1.92×

fold-change

GGT-driven free γ-glutamyl peptide amplification fold-change in Allium sativum

Technical Specifications

SKU

SS-F-KOK-893

Genomic Edits

GC Content

44.6%

Construct Length

2,763 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-4000

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

192

Pathway Edits

GGT1 (γ-glutamyl transpeptidase 1) catalytic activity optimization via active-site engineering
GGT4-high (high-expression GGT4 isoform) overexpression cassette for parallel transfer pathway
GGT-VAC (vacuolar GGT isoform) expression for substrate mobilization from vacuolar GSH pool
AA-perm (amino acid permease) upregulation for acceptor substrate import and GGT reaction rate
Glutamate donor pool expansion via GS feedback-loop attenuation
Transpeptidase specificity maturation for preferred acceptor amino acid bias toward CaSR ligands

Key Interventions

GGT1/GGT4-high dual-isoform overexpression for high-flux γ-glutamyl transpeptidation producing diverse CaSR ligandsGGT-VAC vacuolar isoform expression for mobilization of vacuolar GSH pool into free γ-glutamyl peptide productsAA-perm acceptor substrate import upregulation for maximum GGT reaction rate and product diversityProtoplast PEG-fusion delivery optimized for Allium sativum genome accessibilityTier-II construct validated with 4 precision edits for 1.92× fold-change

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

192

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

GGT-amplified garlic kokumi foundation for pilot broth and stock formulation development

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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