Mouthfeel Enhancement V-IV-A

Glycine max

Soybean

Tier VMouthfeel Enhancement

Target Trait

4.5×

fold-change

Kokumi mouthcoating composite fold-change in Glycine max

Technical Specifications

SKU

SS-F-KOK-879

Genomic Edits

GC Content

45.3%

Construct Length

4,718 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

450

Pathway Edits

GCL (γ-glutamylcysteine ligase) overexpression for GSH mouthcoat foundation biosynthesis
GS (glutathione synthetase) expression for complete GSH scaffold production in soybean
proteinase-B expression for high-molecular-weight peptide liberation from storage proteins
LAP (leucine aminopeptidase) activity optimization for peptide trimming to CaSR-active length
GGT (γ-glutamyl transpeptidase) expression for γ-glutamyl decoration of mouthcoat peptides
Mouthcoating protein scaffold optimization via 7S globulin promoter enhancement
Peptide matrix crosslinking enzyme expression for high-viscosity kokumi mouthcoating

Key Interventions

GCL/GS GSH production for mouthcoat foundation combined with soybean storage protein scaffoldproteinase-B/LAP co-expression for high-MW peptide liberation and trimming to CaSR-active sizesGGT γ-glutamyl decoration of liberated peptides for simultaneous mouthcoating and CaSR engagementCell-free in vitro transcription/translation delivery optimized for Glycine max genome accessibilityTier-V construct validated with 9 precision edits for 4.5× fold-change

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

450

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Ultra-premium kokumi mouthfeel ingredient for luxury plant-based and culinary product lines

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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