IFI-K · Mouthfeel Enhancement
Specimen SS-F-KOK-854 // KOKUMI · IFI-K
Mouthfeel Enhancement II-PR-B
Glycine max
Soybean
Tier IIMouthfeel Enhancement
Target Trait
2.52×
fold-change
Kokumi mouthcoating composite fold-change in Glycine max
Technical Specifications
SKU
SS-F-KOK-854
Genomic Edits
6
GC Content
46.8%
Construct Length
3,660 bp
Promoter System
Ubiquitin / T7
Expression System
Protoplast PEG-fusion
Host Strain
PEG-4000
Receptor Target
CaSR (calcium-sensing receptor)
IFI Score
252
Pathway Edits
- GCL (γ-glutamylcysteine ligase) overexpression for GSH mouthcoat foundation biosynthesis
- GS (glutathione synthetase) expression for complete GSH scaffold production in soybean
- proteinase-B expression for high-molecular-weight peptide liberation from storage proteins
- LAP (leucine aminopeptidase) activity optimization for peptide trimming to CaSR-active length
- GGT (γ-glutamyl transpeptidase) expression for γ-glutamyl decoration of mouthcoat peptides
- Mouthcoating protein scaffold optimization via 7S globulin promoter enhancement
- Peptide matrix crosslinking enzyme expression for high-viscosity kokumi mouthcoating
Key Interventions
GCL/GS GSH production for mouthcoat foundation combined with soybean storage protein scaffoldproteinase-B/LAP co-expression for high-MW peptide liberation and trimming to CaSR-active sizesGGT γ-glutamyl decoration of liberated peptides for simultaneous mouthcoating and CaSR engagementProtoplast PEG-fusion delivery optimized for Glycine max genome accessibilityTier-II construct validated with 6 precision edits for 2.52× fold-change
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K252
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
Soy-derived mouthfeel kokumi foundation for pilot plant-based meat and dairy analog formulations
License
$749 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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