IFI-K · Mouthfeel Enhancement

Specimen SS-F-KOK-847 // KOKUMI · IFI-K

Mouthfeel Enhancement I-IV-A

Glycine max

Soybean

Tier IMouthfeel Enhancement

Target Trait

1.6×

fold-change

Kokumi mouthcoating composite fold-change in Glycine max

Technical Specifications

SKU

SS-F-KOK-847

Genomic Edits

4

GC Content

44.7%

Construct Length

2,750 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

160

Pathway Edits

  • GCL (γ-glutamylcysteine ligase) overexpression for GSH mouthcoat foundation biosynthesis
  • GS (glutathione synthetase) expression for complete GSH scaffold production in soybean
  • proteinase-B expression for high-molecular-weight peptide liberation from storage proteins
  • LAP (leucine aminopeptidase) activity optimization for peptide trimming to CaSR-active length
  • GGT (γ-glutamyl transpeptidase) expression for γ-glutamyl decoration of mouthcoat peptides
  • Mouthcoating protein scaffold optimization via 7S globulin promoter enhancement
  • Peptide matrix crosslinking enzyme expression for high-viscosity kokumi mouthcoating

Key Interventions

GCL/GS GSH production for mouthcoat foundation combined with soybean storage protein scaffoldproteinase-B/LAP co-expression for high-MW peptide liberation and trimming to CaSR-active sizesGGT γ-glutamyl decoration of liberated peptides for simultaneous mouthcoating and CaSR engagementCell-free in vitro transcription/translation delivery optimized for Glycine max genome accessibilityTier-I construct validated with 4 precision edits for 1.6× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
160
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Research-grade mouthfeel-kokumi reference ingredient for mouthcoating mechanism studies

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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