IFI-K · Mouthfeel Enhancement
Specimen SS-F-KOK-845 // KOKUMI · IFI-K
Mouthfeel Enhancement I-PR-A
Glycine max
Soybean
Tier IMouthfeel Enhancement
Target Trait
1.6×
fold-change
Kokumi mouthcoating composite fold-change in Glycine max
Technical Specifications
SKU
SS-F-KOK-845
Genomic Edits
4
GC Content
43.9%
Construct Length
2,916 bp
Promoter System
Ubiquitin / T7
Expression System
Protoplast PEG-fusion
Host Strain
PEG-4000
Receptor Target
CaSR (calcium-sensing receptor)
IFI Score
160
Pathway Edits
- GCL (γ-glutamylcysteine ligase) overexpression for GSH mouthcoat foundation biosynthesis
- GS (glutathione synthetase) expression for complete GSH scaffold production in soybean
- proteinase-B expression for high-molecular-weight peptide liberation from storage proteins
- LAP (leucine aminopeptidase) activity optimization for peptide trimming to CaSR-active length
- GGT (γ-glutamyl transpeptidase) expression for γ-glutamyl decoration of mouthcoat peptides
- Mouthcoating protein scaffold optimization via 7S globulin promoter enhancement
- Peptide matrix crosslinking enzyme expression for high-viscosity kokumi mouthcoating
Key Interventions
GCL/GS GSH production for mouthcoat foundation combined with soybean storage protein scaffoldproteinase-B/LAP co-expression for high-MW peptide liberation and trimming to CaSR-active sizesGGT γ-glutamyl decoration of liberated peptides for simultaneous mouthcoating and CaSR engagementProtoplast PEG-fusion delivery optimized for Glycine max genome accessibilityTier-I construct validated with 4 precision edits for 1.6× fold-change
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K160
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
Research-grade mouthfeel-kokumi reference ingredient for mouthcoating mechanism studies
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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