Mouthfeel Enhancement I-BL-B

Glycine max

Soybean

Tier IMouthfeel Enhancement

Target Trait

1.73×

fold-change

Kokumi mouthcoating composite fold-change in Glycine max

Technical Specifications

SKU

SS-F-KOK-844

Genomic Edits

GC Content

47.1%

Construct Length

3,279 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle delivery

Host Strain

0.6µm Gold Particle

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

173

Pathway Edits

GCL (γ-glutamylcysteine ligase) overexpression for GSH mouthcoat foundation biosynthesis
GS (glutathione synthetase) expression for complete GSH scaffold production in soybean
proteinase-B expression for high-molecular-weight peptide liberation from storage proteins
LAP (leucine aminopeptidase) activity optimization for peptide trimming to CaSR-active length
GGT (γ-glutamyl transpeptidase) expression for γ-glutamyl decoration of mouthcoat peptides
Mouthcoating protein scaffold optimization via 7S globulin promoter enhancement
Peptide matrix crosslinking enzyme expression for high-viscosity kokumi mouthcoating

Key Interventions

GCL/GS GSH production for mouthcoat foundation combined with soybean storage protein scaffoldproteinase-B/LAP co-expression for high-MW peptide liberation and trimming to CaSR-active sizesGGT γ-glutamyl decoration of liberated peptides for simultaneous mouthcoating and CaSR engagementBiolistic particle delivery delivery optimized for Glycine max genome accessibilityTier-I construct validated with 5 precision edits for 1.73× fold-change

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

173

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Research-grade mouthfeel-kokumi reference ingredient for mouthcoating mechanism studies

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Kokumi · IFI-K All Modalities