IFI-K · Vanilla-Kokumi
Specimen SS-F-KOK-840 // KOKUMI · IFI-K
Vanilla-Kokumi V-IV-B
Vanilla planifolia
Vanilla
Tier VVanilla-Kokumi
Target Trait
3.24×
fold-change
Vanilla phenylpropanoid CaSR modulator fold-change in Vanilla planifolia
Technical Specifications
SKU
SS-F-KOK-840
Genomic Edits
8
GC Content
47.4%
Construct Length
4,275 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free in vitro transcription/translation
Host Strain
T7 RNA Polymerase
Receptor Target
CaSR (calcium-sensing receptor)
IFI Score
324
Pathway Edits
- VAMT (vanillin O-methyltransferase) activity optimization for phenylpropanoid CaSR ligand methylation
- 4CL (4-coumarate:CoA ligase) expression for phenylpropanoid flux entry-point activation
- C3H (coumaroylquinate 3-hydroxylase) expression for catechol ring formation in vanilla pathway
- COMT (caffeate O-methyltransferase) expression for methoxyphenol CaSR ligand decoration
- Peroxidase-coupled oxidation pathway for vanillin analog CaSR modulator final step
- Phenylpropanoid flux enhancement via PAL (phenylalanine ammonia lyase) upregulation
Key Interventions
VAMT/COMT methylation pathway optimization for phenylpropanoid CaSR allosteric modulator decoration4CL/C3H phenylpropanoid flux activation for vanilla-specific CaSR kokumi compound productionPeroxidase-coupled vanillin analog biosynthesis for CaSR extracellular binding site engagementCell-free in vitro transcription/translation delivery optimized for Vanilla planifolia genome accessibilityTier-V construct validated with 8 precision edits for 3.24× fold-change
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K324
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
Ultra-premium vanilla kokumi isolate for luxury confectionery and high-end dessert lines
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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