IFI-K · Vanilla-Kokumi

Specimen SS-F-KOK-838 // KOKUMI · IFI-K

Vanilla-Kokumi V-PR-B

Vanilla planifolia

Vanilla

Tier VVanilla-Kokumi

Target Trait

3.24×

fold-change

Vanilla phenylpropanoid CaSR modulator fold-change in Vanilla planifolia

Technical Specifications

SKU

SS-F-KOK-838

Genomic Edits

8

GC Content

46.9%

Construct Length

4,241 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-4000

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

324

Pathway Edits

  • VAMT (vanillin O-methyltransferase) activity optimization for phenylpropanoid CaSR ligand methylation
  • 4CL (4-coumarate:CoA ligase) expression for phenylpropanoid flux entry-point activation
  • C3H (coumaroylquinate 3-hydroxylase) expression for catechol ring formation in vanilla pathway
  • COMT (caffeate O-methyltransferase) expression for methoxyphenol CaSR ligand decoration
  • Peroxidase-coupled oxidation pathway for vanillin analog CaSR modulator final step
  • Phenylpropanoid flux enhancement via PAL (phenylalanine ammonia lyase) upregulation

Key Interventions

VAMT/COMT methylation pathway optimization for phenylpropanoid CaSR allosteric modulator decoration4CL/C3H phenylpropanoid flux activation for vanilla-specific CaSR kokumi compound productionPeroxidase-coupled vanillin analog biosynthesis for CaSR extracellular binding site engagementProtoplast PEG-fusion delivery optimized for Vanilla planifolia genome accessibilityTier-V construct validated with 8 precision edits for 3.24× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
324
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Ultra-premium vanilla kokumi isolate for luxury confectionery and high-end dessert lines

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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