IFI-K · Vanilla-Kokumi
Specimen SS-F-KOK-832 // KOKUMI · IFI-K
Vanilla-Kokumi IV-IV-B
Vanilla planifolia
Vanilla
Tier IVVanilla-Kokumi
Target Trait
2.75×
fold-change
Vanilla phenylpropanoid CaSR modulator fold-change in Vanilla planifolia
Technical Specifications
SKU
SS-F-KOK-832
Genomic Edits
7
GC Content
46.2%
Construct Length
3,928 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free in vitro transcription/translation
Host Strain
T7 RNA Polymerase
Receptor Target
CaSR (calcium-sensing receptor)
IFI Score
275
Pathway Edits
- VAMT (vanillin O-methyltransferase) activity optimization for phenylpropanoid CaSR ligand methylation
- 4CL (4-coumarate:CoA ligase) expression for phenylpropanoid flux entry-point activation
- C3H (coumaroylquinate 3-hydroxylase) expression for catechol ring formation in vanilla pathway
- COMT (caffeate O-methyltransferase) expression for methoxyphenol CaSR ligand decoration
- Peroxidase-coupled oxidation pathway for vanillin analog CaSR modulator final step
- Phenylpropanoid flux enhancement via PAL (phenylalanine ammonia lyase) upregulation
Key Interventions
VAMT/COMT methylation pathway optimization for phenylpropanoid CaSR allosteric modulator decoration4CL/C3H phenylpropanoid flux activation for vanilla-specific CaSR kokumi compound productionPeroxidase-coupled vanillin analog biosynthesis for CaSR extracellular binding site engagementCell-free in vitro transcription/translation delivery optimized for Vanilla planifolia genome accessibilityTier-IV construct validated with 7 precision edits for 2.75× fold-change
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K275
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
Premium vanilla CaSR modulator ingredient for patisserie and fine-chocolate applications
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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