Vanilla-Kokumi III-IV-B

Vanilla planifolia

Vanilla

Tier IIIVanilla-Kokumi

Target Trait

2.27×

fold-change

Vanilla phenylpropanoid CaSR modulator fold-change in Vanilla planifolia

Technical Specifications

SKU

SS-F-KOK-824

Genomic Edits

GC Content

48.9%

Construct Length

3,581 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

227

Pathway Edits

VAMT (vanillin O-methyltransferase) activity optimization for phenylpropanoid CaSR ligand methylation
4CL (4-coumarate:CoA ligase) expression for phenylpropanoid flux entry-point activation
C3H (coumaroylquinate 3-hydroxylase) expression for catechol ring formation in vanilla pathway
COMT (caffeate O-methyltransferase) expression for methoxyphenol CaSR ligand decoration
Peroxidase-coupled oxidation pathway for vanillin analog CaSR modulator final step
Phenylpropanoid flux enhancement via PAL (phenylalanine ammonia lyase) upregulation

Key Interventions

VAMT/COMT methylation pathway optimization for phenylpropanoid CaSR allosteric modulator decoration4CL/C3H phenylpropanoid flux activation for vanilla-specific CaSR kokumi compound productionPeroxidase-coupled vanillin analog biosynthesis for CaSR extracellular binding site engagementCell-free in vitro transcription/translation delivery optimized for Vanilla planifolia genome accessibilityTier-III construct validated with 6 precision edits for 2.27× fold-change

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

227

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Commercial vanilla kokumi extract for premium dessert, chocolate and confectionery manufacture

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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