IFI-K · Vanilla-Kokumi

Specimen SS-F-KOK-807 // KOKUMI · IFI-K

Vanilla-Kokumi I-IV-A

Vanilla planifolia

Vanilla

Tier IVanilla-Kokumi

Target Trait

1.2×

fold-change

Vanilla phenylpropanoid CaSR modulator fold-change in Vanilla planifolia

Technical Specifications

SKU

SS-F-KOK-807

Genomic Edits

3

GC Content

44.7%

Construct Length

2,490 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

120

Pathway Edits

  • VAMT (vanillin O-methyltransferase) activity optimization for phenylpropanoid CaSR ligand methylation
  • 4CL (4-coumarate:CoA ligase) expression for phenylpropanoid flux entry-point activation
  • C3H (coumaroylquinate 3-hydroxylase) expression for catechol ring formation in vanilla pathway
  • COMT (caffeate O-methyltransferase) expression for methoxyphenol CaSR ligand decoration
  • Peroxidase-coupled oxidation pathway for vanillin analog CaSR modulator final step
  • Phenylpropanoid flux enhancement via PAL (phenylalanine ammonia lyase) upregulation

Key Interventions

VAMT/COMT methylation pathway optimization for phenylpropanoid CaSR allosteric modulator decoration4CL/C3H phenylpropanoid flux activation for vanilla-specific CaSR kokumi compound productionPeroxidase-coupled vanillin analog biosynthesis for CaSR extracellular binding site engagementCell-free in vitro transcription/translation delivery optimized for Vanilla planifolia genome accessibilityTier-I construct validated with 3 precision edits for 1.2× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
120
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Research-grade vanilla phenylpropanoid CaSR modulator for aromatic kokumi mechanism studies

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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