IFI-K · CaSR EC50 Optimization

Specimen SS-F-KOK-800 // KOKUMI · IFI-K

CaSR EC50 Optimization V-IV-B

Allium sativum

Garlic

Tier VCaSR EC50 Optimization

Target Trait

1.38

µM EC50

CaSR agonist potency (EC50 µM) — lower is more potent

Technical Specifications

SKU

SS-F-KOK-800

Genomic Edits

10

GC Content

47.4%

Construct Length

5,155 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

724.6

Pathway Edits

  • GCL-opt (kinetics-tuned GCL variant) expression for sub-saturation GSH biosynthesis optimization
  • GS-opt (high-affinity GS variant) expression with improved cysteinylglycine Km engineering
  • GGT-kinetics activity-optimized GGT expression for high-turnover γ-glutamyl transfer
  • SAT-kinetics (kinetics-optimized serine acetyltransferase) rate-limiting step removal
  • CaSR agonist delivery cassette for sub-micromolar EC50 kokumi compound channeling
  • Metabolic channeling scaffold for direct enzyme-to-CaSR ligand substrate transfer
  • Substrate import transporter expression for precursor saturation in low-concentration regime

Key Interventions

GCL-opt/GS-opt kinetics-tuned GSH production for sub-micromolar CaSR EC50 agonist concentration achievementGGT-kinetics transpeptidase rate optimization for high-turnover γ-glutamyl transfer at CaSR-saturating ratesSAT-kinetics substrate channeling for removal of rate-limiting steps achieving lowest-possible CaSR EC50Cell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-V construct validated with 10 precision edits for 1.38 µM EC50

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
724.6
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Ultra-premium minimum-EC50 CaSR agonist for luxury kokumi micro-dosing product lines

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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