CaSR EC50 Optimization V-AG-A

Allium sativum

Garlic

Tier VCaSR EC50 Optimization

Target Trait

1.5

µM EC50

CaSR agonist potency (EC50 µM) — lower is more potent

Technical Specifications

SKU

SS-F-KOK-793

Genomic Edits

GC Content

45.9%

Construct Length

4,656 bp

Promoter System

Tissue-specific 2A11 / CaMV 35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

666.7

Pathway Edits

GCL-opt (kinetics-tuned GCL variant) expression for sub-saturation GSH biosynthesis optimization
GS-opt (high-affinity GS variant) expression with improved cysteinylglycine Km engineering
GGT-kinetics activity-optimized GGT expression for high-turnover γ-glutamyl transfer
SAT-kinetics (kinetics-optimized serine acetyltransferase) rate-limiting step removal
CaSR agonist delivery cassette for sub-micromolar EC50 kokumi compound channeling
Metabolic channeling scaffold for direct enzyme-to-CaSR ligand substrate transfer
Substrate import transporter expression for precursor saturation in low-concentration regime

Key Interventions

GCL-opt/GS-opt kinetics-tuned GSH production for sub-micromolar CaSR EC50 agonist concentration achievementGGT-kinetics transpeptidase rate optimization for high-turnover γ-glutamyl transfer at CaSR-saturating ratesSAT-kinetics substrate channeling for removal of rate-limiting steps achieving lowest-possible CaSR EC50Agrobacterium-mediated T-DNA delivery optimized for Allium sativum genome accessibilityTier-V construct validated with 9 precision edits for 1.5 µM EC50

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

666.7

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Ultra-premium minimum-EC50 CaSR agonist for luxury kokumi micro-dosing product lines

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Kokumi · IFI-K All Modalities