IFI-K · CaSR EC50 Optimization

Specimen SS-F-KOK-786 // KOKUMI · IFI-K

CaSR EC50 Optimization IV-AG-B

Allium sativum

Garlic

Tier IVCaSR EC50 Optimization

Target Trait

1.96

µM EC50

CaSR agonist potency (EC50 µM) — lower is more potent

Technical Specifications

SKU

SS-F-KOK-786

Genomic Edits

9

GC Content

46.7%

Construct Length

4,706 bp

Promoter System

Tissue-specific 2A11 / CaMV 35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

510.2

Pathway Edits

  • GCL-opt (kinetics-tuned GCL variant) expression for sub-saturation GSH biosynthesis optimization
  • GS-opt (high-affinity GS variant) expression with improved cysteinylglycine Km engineering
  • GGT-kinetics activity-optimized GGT expression for high-turnover γ-glutamyl transfer
  • SAT-kinetics (kinetics-optimized serine acetyltransferase) rate-limiting step removal
  • CaSR agonist delivery cassette for sub-micromolar EC50 kokumi compound channeling
  • Metabolic channeling scaffold for direct enzyme-to-CaSR ligand substrate transfer
  • Substrate import transporter expression for precursor saturation in low-concentration regime

Key Interventions

GCL-opt/GS-opt kinetics-tuned GSH production for sub-micromolar CaSR EC50 agonist concentration achievementGGT-kinetics transpeptidase rate optimization for high-turnover γ-glutamyl transfer at CaSR-saturating ratesSAT-kinetics substrate channeling for removal of rate-limiting steps achieving lowest-possible CaSR EC50Agrobacterium-mediated T-DNA delivery optimized for Allium sativum genome accessibilityTier-IV construct validated with 9 precision edits for 1.96 µM EC50

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
510.2
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium ultra-low-dose CaSR agonist ingredient for restaurant and specialty culinary applications

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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