CaSR EC50 Optimization III-PR-B

Allium sativum

Garlic

Tier IIICaSR EC50 Optimization

Target Trait

2.53

µM EC50

CaSR agonist potency (EC50 µM) — lower is more potent

Technical Specifications

SKU

SS-F-KOK-782

Genomic Edits

GC Content

47.6%

Construct Length

4,427 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-4000

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

395.3

Pathway Edits

GCL-opt (kinetics-tuned GCL variant) expression for sub-saturation GSH biosynthesis optimization
GS-opt (high-affinity GS variant) expression with improved cysteinylglycine Km engineering
GGT-kinetics activity-optimized GGT expression for high-turnover γ-glutamyl transfer
SAT-kinetics (kinetics-optimized serine acetyltransferase) rate-limiting step removal
CaSR agonist delivery cassette for sub-micromolar EC50 kokumi compound channeling
Metabolic channeling scaffold for direct enzyme-to-CaSR ligand substrate transfer
Substrate import transporter expression for precursor saturation in low-concentration regime

Key Interventions

GCL-opt/GS-opt kinetics-tuned GSH production for sub-micromolar CaSR EC50 agonist concentration achievementGGT-kinetics transpeptidase rate optimization for high-turnover γ-glutamyl transfer at CaSR-saturating ratesSAT-kinetics substrate channeling for removal of rate-limiting steps achieving lowest-possible CaSR EC50Protoplast PEG-fusion delivery optimized for Allium sativum genome accessibilityTier-III construct validated with 8 precision edits for 2.53 µM EC50

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

395.3

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Commercial ultra-potent kokumi extract for high-efficiency sauce and broth manufacture

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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