IFI-K · Homoglutathione

Specimen SS-F-KOK-744 // KOKUMI · IFI-K

Homoglutathione III-IV-B

Glycine max

Soybean

Tier IIIHomoglutathione

Target Trait

2.59×

fold-change

Homoglutathione (γ-Glu-Cys-β-Ala) fold-change in Glycine max

Technical Specifications

SKU

SS-F-KOK-744

Genomic Edits

6

GC Content

48.9%

Construct Length

3,621 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

259

Pathway Edits

  • HGSL (homoglutathione synthetase-like) expression for γ-Glu-Cys-β-Ala tripeptide biosynthesis
  • HPMS (hydroxypropylmalonyl-ACP synthase homolog) expression for β-alanine pathway activation
  • GCL-alt (alternative GCL isoform) expression for γ-Glu-Cys dipeptide scaffold supply
  • Gly-I-alt (glyoxalase I alternative) modulation to redirect tripeptide synthesis toward hGSH
  • Vacuolar hGSH transporter upregulation for cytosolic homoglutathione pool expansion
  • Beta-alanine aminotransferase expression for β-alanine precursor supply to HGSL

Key Interventions

HGSL/HPMS homoglutathione pathway expression for legume-specific CaSR γ-glutamyl tripeptide productionGCL-alt γ-Glu-Cys dipeptide scaffold supply for HGSL-mediated β-alanyl extension to hGSHVacuolar hGSH export transporter upregulation for cytosolic accumulation of CaSR kokumi ligandCell-free in vitro transcription/translation delivery optimized for Glycine max genome accessibilityTier-III construct validated with 6 precision edits for 2.59× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
259
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial soy homoglutathione extract for sauce, condiment and savory ingredient manufacture

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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