IFI-K · Sulfur Amino Acid Kokumi

Specimen SS-F-KOK-720 // KOKUMI · IFI-K

Sulfur Amino Acid Kokumi V-IV-B

Allium sativum

Garlic

Tier VSulfur Amino Acid Kokumi

Target Trait

4.1×

fold-change

Cysteine/methionine-based CaSR ligand pool fold-change in Allium sativum

Technical Specifications

SKU

SS-F-KOK-720

Genomic Edits

9

GC Content

47.4%

Construct Length

4,815 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

410

Pathway Edits

  • OAS-TL (O-acetylserine thiol-lyase) overexpression for cysteine biosynthesis from serine and sulfide
  • SAT1 (serine acetyltransferase 1) expression for O-acetylserine cysteine precursor supply
  • ACS1 (1-aminocyclopropane-1-carboxylate synthase 1) rerouting for S-allyl cysteine biosynthesis
  • MGL (methionine gamma-lyase) expression for methionine-derived CaSR ligand conversion
  • gamma-ECS intermediate pathway for cysteine-to-γ-glutamyl-cysteine flux optimization
  • Cysteine sulfoxide pool expansion via FMOX sulfoxidation activity enhancement

Key Interventions

OAS-TL/SAT1 cysteine biosynthesis pathway for CaSR sulfur amino acid ligand precursor supplyACS1 S-allyl channeling and MGL methionine lyase for diverse sulfur CaSR ligand productiongamma-ECS intermediate optimization for cysteine-to-γ-glutamyl-cysteine kokumi substrate fluxCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-V construct validated with 9 precision edits for 4.1× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
410
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Ultra-premium sulfur amino acid kokumi for luxury garlic-forward product lines

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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