IFI-K · Sulfur Amino Acid Kokumi
Specimen SS-F-KOK-712 // KOKUMI · IFI-K
Sulfur Amino Acid Kokumi IV-IV-B
Allium sativum
Garlic
Tier IVSulfur Amino Acid Kokumi
Target Trait
3.46×
fold-change
Cysteine/methionine-based CaSR ligand pool fold-change in Allium sativum
Technical Specifications
SKU
SS-F-KOK-712
Genomic Edits
8
GC Content
46.2%
Construct Length
4,268 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free in vitro transcription/translation
Host Strain
T7 RNA Polymerase
Receptor Target
CaSR (calcium-sensing receptor)
IFI Score
346
Pathway Edits
- OAS-TL (O-acetylserine thiol-lyase) overexpression for cysteine biosynthesis from serine and sulfide
- SAT1 (serine acetyltransferase 1) expression for O-acetylserine cysteine precursor supply
- ACS1 (1-aminocyclopropane-1-carboxylate synthase 1) rerouting for S-allyl cysteine biosynthesis
- MGL (methionine gamma-lyase) expression for methionine-derived CaSR ligand conversion
- gamma-ECS intermediate pathway for cysteine-to-γ-glutamyl-cysteine flux optimization
- Cysteine sulfoxide pool expansion via FMOX sulfoxidation activity enhancement
Key Interventions
OAS-TL/SAT1 cysteine biosynthesis pathway for CaSR sulfur amino acid ligand precursor supplyACS1 S-allyl channeling and MGL methionine lyase for diverse sulfur CaSR ligand productiongamma-ECS intermediate optimization for cysteine-to-γ-glutamyl-cysteine kokumi substrate fluxCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-IV construct validated with 8 precision edits for 3.46× fold-change
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K346
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
Premium garlic sulfur kokumi ingredient for restaurant and specialty culinary applications
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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