IFI-K · Sulfur Amino Acid Kokumi

Specimen SS-F-KOK-705 // KOKUMI · IFI-K

Sulfur Amino Acid Kokumi IV-AG-A

Allium sativum

Garlic

Tier IVSulfur Amino Acid Kokumi

Target Trait

3.2×

fold-change

Cysteine/methionine-based CaSR ligand pool fold-change in Allium sativum

Technical Specifications

SKU

SS-F-KOK-705

Genomic Edits

7

GC Content

44.3%

Construct Length

3,969 bp

Promoter System

Tissue-specific 2A11 / CaMV 35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

320

Pathway Edits

  • OAS-TL (O-acetylserine thiol-lyase) overexpression for cysteine biosynthesis from serine and sulfide
  • SAT1 (serine acetyltransferase 1) expression for O-acetylserine cysteine precursor supply
  • ACS1 (1-aminocyclopropane-1-carboxylate synthase 1) rerouting for S-allyl cysteine biosynthesis
  • MGL (methionine gamma-lyase) expression for methionine-derived CaSR ligand conversion
  • gamma-ECS intermediate pathway for cysteine-to-γ-glutamyl-cysteine flux optimization
  • Cysteine sulfoxide pool expansion via FMOX sulfoxidation activity enhancement

Key Interventions

OAS-TL/SAT1 cysteine biosynthesis pathway for CaSR sulfur amino acid ligand precursor supplyACS1 S-allyl channeling and MGL methionine lyase for diverse sulfur CaSR ligand productiongamma-ECS intermediate optimization for cysteine-to-γ-glutamyl-cysteine kokumi substrate fluxAgrobacterium-mediated T-DNA delivery optimized for Allium sativum genome accessibilityTier-IV construct validated with 7 precision edits for 3.2× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
320
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium garlic sulfur kokumi ingredient for restaurant and specialty culinary applications

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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