Glutamyl Dipeptide Library IV-AG-B

Allium sativum

Garlic

Tier IVGlutamyl Dipeptide Library

Target Trait

4.07×

fold-change

Broad-spectrum γ-glutamyl dipeptide library CaSR occupancy fold-change

Technical Specifications

SKU

SS-F-KOK-666

Genomic Edits

GC Content

46.7%

Construct Length

4,666 bp

Promoter System

Tissue-specific 2A11 / CaMV 35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

407

Pathway Edits

GGT1 broad-specificity active-site engineering for diverse amino acid acceptor substrate tolerance
GCL substrate supply amplification for γ-glutamyl donor pool expansion
ALT competitive sink silencing to redirect glutamate toward GGT catalysis
AlaAT2 (alanine aminotransferase 2) expression for β-amino acid acceptor pathway activation
GGT4 high-specificity isoform expression for Val/Leu/Ile acceptor dipeptide synthesis
Glutamate transporter upregulation for sustained γ-glutamyl donor substrate supply
Dipeptide ABC exporter engineering for extracellular library accumulation

Key Interventions

GGT1 broad-specificity engineering and GGT4 high-specificity isoform for diverse γ-glutamyl dipeptide libraryGCL/AlaAT2 substrate supply engineering for high-flux γ-glutamyl donor and acceptor availabilityALT/glutamate transporter competitive drain silencing and supply amplification for CaSR library saturationAgrobacterium-mediated T-DNA delivery optimized for Allium sativum genome accessibilityTier-IV construct validated with 9 precision edits for 4.07× fold-change

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

407

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Premium garlic kokumi library ingredient for restaurant and culinary specialty applications

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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