IFI-K · Glutamyl Dipeptide Library

Specimen SS-F-KOK-664 // KOKUMI · IFI-K

Glutamyl Dipeptide Library III-IV-B

Allium sativum

Garlic

Tier IIIGlutamyl Dipeptide Library

Target Trait

3.29×

fold-change

Broad-spectrum γ-glutamyl dipeptide library CaSR occupancy fold-change

Technical Specifications

SKU

SS-F-KOK-664

Genomic Edits

8

GC Content

48.9%

Construct Length

4,421 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

329

Pathway Edits

  • GGT1 broad-specificity active-site engineering for diverse amino acid acceptor substrate tolerance
  • GCL substrate supply amplification for γ-glutamyl donor pool expansion
  • ALT competitive sink silencing to redirect glutamate toward GGT catalysis
  • AlaAT2 (alanine aminotransferase 2) expression for β-amino acid acceptor pathway activation
  • GGT4 high-specificity isoform expression for Val/Leu/Ile acceptor dipeptide synthesis
  • Glutamate transporter upregulation for sustained γ-glutamyl donor substrate supply
  • Dipeptide ABC exporter engineering for extracellular library accumulation

Key Interventions

GGT1 broad-specificity engineering and GGT4 high-specificity isoform for diverse γ-glutamyl dipeptide libraryGCL/AlaAT2 substrate supply engineering for high-flux γ-glutamyl donor and acceptor availabilityALT/glutamate transporter competitive drain silencing and supply amplification for CaSR library saturationCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-III construct validated with 8 precision edits for 3.29× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
329
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial γ-glutamyl dipeptide library extract for savory condiment and seasoning manufacture

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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