IFI-K · Glutamyl Dipeptide Library

Specimen SS-F-KOK-652 // KOKUMI · IFI-K

Glutamyl Dipeptide Library II-BL-B

Allium sativum

Garlic

Tier IIGlutamyl Dipeptide Library

Target Trait

2.52×

fold-change

Broad-spectrum γ-glutamyl dipeptide library CaSR occupancy fold-change

Technical Specifications

SKU

SS-F-KOK-652

Genomic Edits

6

GC Content

49.1%

Construct Length

3,626 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle delivery

Host Strain

0.6µm Gold Particle

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

252

Pathway Edits

  • GGT1 broad-specificity active-site engineering for diverse amino acid acceptor substrate tolerance
  • GCL substrate supply amplification for γ-glutamyl donor pool expansion
  • ALT competitive sink silencing to redirect glutamate toward GGT catalysis
  • AlaAT2 (alanine aminotransferase 2) expression for β-amino acid acceptor pathway activation
  • GGT4 high-specificity isoform expression for Val/Leu/Ile acceptor dipeptide synthesis
  • Glutamate transporter upregulation for sustained γ-glutamyl donor substrate supply
  • Dipeptide ABC exporter engineering for extracellular library accumulation

Key Interventions

GGT1 broad-specificity engineering and GGT4 high-specificity isoform for diverse γ-glutamyl dipeptide libraryGCL/AlaAT2 substrate supply engineering for high-flux γ-glutamyl donor and acceptor availabilityALT/glutamate transporter competitive drain silencing and supply amplification for CaSR library saturationBiolistic particle delivery delivery optimized for Allium sativum genome accessibilityTier-II construct validated with 6 precision edits for 2.52× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
252
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Garlic-derived broad-spectrum kokumi foundation for pilot sauce and stock formulations

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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