Glutamyl Dipeptide Library II-AG-B

Allium sativum

Garlic

Tier IIGlutamyl Dipeptide Library

Target Trait

2.52×

fold-change

Broad-spectrum γ-glutamyl dipeptide library CaSR occupancy fold-change

Technical Specifications

SKU

SS-F-KOK-650

Genomic Edits

GC Content

47.8%

Construct Length

3,592 bp

Promoter System

Tissue-specific 2A11 / CaMV 35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

252

Pathway Edits

GGT1 broad-specificity active-site engineering for diverse amino acid acceptor substrate tolerance
GCL substrate supply amplification for γ-glutamyl donor pool expansion
ALT competitive sink silencing to redirect glutamate toward GGT catalysis
AlaAT2 (alanine aminotransferase 2) expression for β-amino acid acceptor pathway activation
GGT4 high-specificity isoform expression for Val/Leu/Ile acceptor dipeptide synthesis
Glutamate transporter upregulation for sustained γ-glutamyl donor substrate supply
Dipeptide ABC exporter engineering for extracellular library accumulation

Key Interventions

GGT1 broad-specificity engineering and GGT4 high-specificity isoform for diverse γ-glutamyl dipeptide libraryGCL/AlaAT2 substrate supply engineering for high-flux γ-glutamyl donor and acceptor availabilityALT/glutamate transporter competitive drain silencing and supply amplification for CaSR library saturationAgrobacterium-mediated T-DNA delivery optimized for Allium sativum genome accessibilityTier-II construct validated with 6 precision edits for 2.52× fold-change

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

252

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Garlic-derived broad-spectrum kokumi foundation for pilot sauce and stock formulations

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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