IFI-K · Glutamyl Dipeptide Library
Specimen SS-F-KOK-647 // KOKUMI · IFI-K
Glutamyl Dipeptide Library I-IV-A
Allium sativum
Garlic
Tier IGlutamyl Dipeptide Library
Target Trait
1.6×
fold-change
Broad-spectrum γ-glutamyl dipeptide library CaSR occupancy fold-change
Technical Specifications
SKU
SS-F-KOK-647
Genomic Edits
4
GC Content
44.7%
Construct Length
2,750 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free in vitro transcription/translation
Host Strain
T7 RNA Polymerase
Receptor Target
CaSR (calcium-sensing receptor)
IFI Score
160
Pathway Edits
- GGT1 broad-specificity active-site engineering for diverse amino acid acceptor substrate tolerance
- GCL substrate supply amplification for γ-glutamyl donor pool expansion
- ALT competitive sink silencing to redirect glutamate toward GGT catalysis
- AlaAT2 (alanine aminotransferase 2) expression for β-amino acid acceptor pathway activation
- GGT4 high-specificity isoform expression for Val/Leu/Ile acceptor dipeptide synthesis
- Glutamate transporter upregulation for sustained γ-glutamyl donor substrate supply
- Dipeptide ABC exporter engineering for extracellular library accumulation
Key Interventions
GGT1 broad-specificity engineering and GGT4 high-specificity isoform for diverse γ-glutamyl dipeptide libraryGCL/AlaAT2 substrate supply engineering for high-flux γ-glutamyl donor and acceptor availabilityALT/glutamate transporter competitive drain silencing and supply amplification for CaSR library saturationCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-I construct validated with 4 precision edits for 1.6× fold-change
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K160
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
Research-grade γ-glutamyl dipeptide library for multi-site CaSR occupancy mechanism studies
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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