Glutamyl Dipeptide Library I-BL-A

Allium sativum

Garlic

Tier IGlutamyl Dipeptide Library

Target Trait

1.6×

fold-change

Broad-spectrum γ-glutamyl dipeptide library CaSR occupancy fold-change

Technical Specifications

SKU

SS-F-KOK-643

Genomic Edits

GC Content

46.3%

Construct Length

2,882 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle delivery

Host Strain

0.6µm Gold Particle

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

160

Pathway Edits

GGT1 broad-specificity active-site engineering for diverse amino acid acceptor substrate tolerance
GCL substrate supply amplification for γ-glutamyl donor pool expansion
ALT competitive sink silencing to redirect glutamate toward GGT catalysis
AlaAT2 (alanine aminotransferase 2) expression for β-amino acid acceptor pathway activation
GGT4 high-specificity isoform expression for Val/Leu/Ile acceptor dipeptide synthesis
Glutamate transporter upregulation for sustained γ-glutamyl donor substrate supply
Dipeptide ABC exporter engineering for extracellular library accumulation

Key Interventions

GGT1 broad-specificity engineering and GGT4 high-specificity isoform for diverse γ-glutamyl dipeptide libraryGCL/AlaAT2 substrate supply engineering for high-flux γ-glutamyl donor and acceptor availabilityALT/glutamate transporter competitive drain silencing and supply amplification for CaSR library saturationBiolistic particle delivery delivery optimized for Allium sativum genome accessibilityTier-I construct validated with 4 precision edits for 1.6× fold-change

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

160

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Research-grade γ-glutamyl dipeptide library for multi-site CaSR occupancy mechanism studies

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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