IFI-K · Protein Hydrolysate Kokumi

Specimen SS-F-KOK-615 // KOKUMI · IFI-K

Protein Hydrolysate Kokumi II-IV-A

Glycine max

Soybean

Tier IIProtein Hydrolysate Kokumi

Target Trait

2.13×

fold-change

Kokumi-active protein hydrolysate fold-change in Glycine max

Technical Specifications

SKU

SS-F-KOK-615

Genomic Edits

5

GC Content

45.4%

Construct Length

3,217 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

213

Pathway Edits

  • proteinase-B (vacuolar serine protease) expression for endoprotease-driven protein hydrolysate liberation
  • LAP (leucine aminopeptidase) overexpression for N-terminal amino acid release and peptide trimming
  • CPY (carboxypeptidase Y) expression for C-terminal processing of CaSR-active peptides
  • vacuolar-prot (vacuolar processing enzyme) upregulation for storage protein unlocking
  • GGT (γ-glutamyl transpeptidase) expression for γ-glutamyl decoration of liberated peptides
  • Peptide permease upregulation for cytosolic accumulation of CaSR-active hydrolysate fractions
  • Storage protein 11S globulin promoter swap for high-titre substrate pool expression

Key Interventions

proteinase-B/CPY protease co-expression for endoprotease and exoprotease protein hydrolysate liberationGGT transpeptidase expression to γ-glutamyl-decorate liberated peptides for CaSR kokumi activationPeptide permease upregulation for cytosolic and extracellular CaSR-active hydrolysate accumulationCell-free in vitro transcription/translation delivery optimized for Glycine max genome accessibilityTier-II construct validated with 5 precision edits for 2.13× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
213
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Soy-derived kokumi foundation for pilot broth, stock and savory ingredient formulations

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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