IFI-K · Lanthionine Peptide

Specimen SS-F-KOK-576 // KOKUMI · IFI-K

Lanthionine Peptide II-IV-B

Saccharomyces cerevisiae

Yeast

Tier IILanthionine Peptide

Target Trait

2.11×

fold-change

Cyclic lanthipeptide-analog CaSR ligand fold-change in Saccharomyces cerevisiae

Technical Specifications

SKU

SS-F-KOK-576

Genomic Edits

6

GC Content

47.3%

Construct Length

3,534 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

211

Pathway Edits

  • LANM (lanthipeptide modifying enzyme homolog) activity-tuned expression for Lan dehydration
  • LANB (lanthipeptide biosynthesis protein B) protease expression for leader peptide cleavage
  • LanC-like cyclase expression for lanthionine ring structure formation in yeast
  • ProcA precursor peptide cassette expression with optimized leader sequence
  • Lanthipeptide ABC transporter expression for extracellular cyclic peptide secretion
  • Lan-RG (lanthipeptide regulator/immunity protein) expression for pathway stability
  • Prepeptide processing protease expression for mature cyclic CaSR ligand liberation

Key Interventions

LANM/LANB lanthipeptide modification and cleavage pathway for cyclic CaSR peptide scaffold productionLanC-like cyclase expression for lanthionine ring formation creating conformationally constrained CaSR ligandProcA precursor and ABC transporter for extracellular accumulation of mature cyclic kokumi compoundCell-free in vitro transcription/translation delivery optimized for Saccharomyces cerevisiae genome accessibilityTier-II construct validated with 6 precision edits for 2.11× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
211
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Yeast-platform kokumi foundation for pilot fermented food and condiment applications

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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