IFI-K · Lanthionine Peptide

Specimen SS-F-KOK-572 // KOKUMI · IFI-K

Lanthionine Peptide II-BL-B

Saccharomyces cerevisiae

Yeast

Tier IILanthionine Peptide

Target Trait

2.11×

fold-change

Cyclic lanthipeptide-analog CaSR ligand fold-change in Saccharomyces cerevisiae

Technical Specifications

SKU

SS-F-KOK-572

Genomic Edits

6

GC Content

49.1%

Construct Length

3,666 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle delivery

Host Strain

0.6µm Gold Particle

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

211

Pathway Edits

  • LANM (lanthipeptide modifying enzyme homolog) activity-tuned expression for Lan dehydration
  • LANB (lanthipeptide biosynthesis protein B) protease expression for leader peptide cleavage
  • LanC-like cyclase expression for lanthionine ring structure formation in yeast
  • ProcA precursor peptide cassette expression with optimized leader sequence
  • Lanthipeptide ABC transporter expression for extracellular cyclic peptide secretion
  • Lan-RG (lanthipeptide regulator/immunity protein) expression for pathway stability
  • Prepeptide processing protease expression for mature cyclic CaSR ligand liberation

Key Interventions

LANM/LANB lanthipeptide modification and cleavage pathway for cyclic CaSR peptide scaffold productionLanC-like cyclase expression for lanthionine ring formation creating conformationally constrained CaSR ligandProcA precursor and ABC transporter for extracellular accumulation of mature cyclic kokumi compoundBiolistic particle delivery delivery optimized for Saccharomyces cerevisiae genome accessibilityTier-II construct validated with 6 precision edits for 2.11× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
211
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Yeast-platform kokumi foundation for pilot fermented food and condiment applications

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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