IFI-K · Betaine Kokumi

Specimen SS-F-KOK-542 // KOKUMI · IFI-K

Betaine Kokumi III-PR-B

Capsicum annuum

Pepper

Tier IIIBetaine Kokumi

Target Trait

1.89×

fold-change

Glycine betaine CaSR interaction fold-change in Capsicum annuum

Technical Specifications

SKU

SS-F-KOK-542

Genomic Edits

6

GC Content

47.6%

Construct Length

3,587 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-4000

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

189

Pathway Edits

  • BADH (betaine aldehyde dehydrogenase) overexpression for choline-to-betaine oxidation
  • CMOB (choline monooxygenase B) expression for betaine aldehyde intermediate generation
  • CMO (choline monooxygenase) upregulation for choline oxidation pathway activation
  • BCSA (betaine choline sodium antiporter) upregulation for cytosolic betaine accumulation
  • Betaine-GABA transporter-like protein expression for extracellular betaine release

Key Interventions

CMO/BADH two-step betaine biosynthesis from choline for CaSR allosteric modulator accumulationBCSA antiporter upregulation for cytosolic betaine pool expansion in Capsicum annuumBetaine transport expression for extracellular release and CaSR allosteric site accessProtoplast PEG-fusion delivery optimized for Capsicum annuum genome accessibilityTier-III construct validated with 6 precision edits for 1.89× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
189
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial betaine-enriched Capsicum extract for sauce, broth and condiment manufacture

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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