IFI-K · Betaine Kokumi

Specimen SS-F-KOK-540 // KOKUMI · IFI-K

Betaine Kokumi III-BL-B

Capsicum annuum

Pepper

Tier IIIBetaine Kokumi

Target Trait

1.89×

fold-change

Glycine betaine CaSR interaction fold-change in Capsicum annuum

Technical Specifications

SKU

SS-F-KOK-540

Genomic Edits

6

GC Content

46.5%

Construct Length

3,553 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle delivery

Host Strain

0.6µm Gold Particle

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

189

Pathway Edits

  • BADH (betaine aldehyde dehydrogenase) overexpression for choline-to-betaine oxidation
  • CMOB (choline monooxygenase B) expression for betaine aldehyde intermediate generation
  • CMO (choline monooxygenase) upregulation for choline oxidation pathway activation
  • BCSA (betaine choline sodium antiporter) upregulation for cytosolic betaine accumulation
  • Betaine-GABA transporter-like protein expression for extracellular betaine release

Key Interventions

CMO/BADH two-step betaine biosynthesis from choline for CaSR allosteric modulator accumulationBCSA antiporter upregulation for cytosolic betaine pool expansion in Capsicum annuumBetaine transport expression for extracellular release and CaSR allosteric site accessBiolistic particle delivery delivery optimized for Capsicum annuum genome accessibilityTier-III construct validated with 6 precision edits for 1.89× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
189
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial betaine-enriched Capsicum extract for sauce, broth and condiment manufacture

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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