IFI-K · Betaine Kokumi

Specimen SS-F-KOK-538 // KOKUMI · IFI-K

Betaine Kokumi III-AG-B

Capsicum annuum

Pepper

Tier IIIBetaine Kokumi

Target Trait

1.89×

fold-change

Glycine betaine CaSR interaction fold-change in Capsicum annuum

Technical Specifications

SKU

SS-F-KOK-538

Genomic Edits

6

GC Content

48.2%

Construct Length

3,519 bp

Promoter System

Tissue-specific 2A11 / CaMV 35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

189

Pathway Edits

  • BADH (betaine aldehyde dehydrogenase) overexpression for choline-to-betaine oxidation
  • CMOB (choline monooxygenase B) expression for betaine aldehyde intermediate generation
  • CMO (choline monooxygenase) upregulation for choline oxidation pathway activation
  • BCSA (betaine choline sodium antiporter) upregulation for cytosolic betaine accumulation
  • Betaine-GABA transporter-like protein expression for extracellular betaine release

Key Interventions

CMO/BADH two-step betaine biosynthesis from choline for CaSR allosteric modulator accumulationBCSA antiporter upregulation for cytosolic betaine pool expansion in Capsicum annuumBetaine transport expression for extracellular release and CaSR allosteric site accessAgrobacterium-mediated T-DNA delivery optimized for Capsicum annuum genome accessibilityTier-III construct validated with 6 precision edits for 1.89× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
189
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial betaine-enriched Capsicum extract for sauce, broth and condiment manufacture

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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