IFI-K · Betaine Kokumi

Specimen SS-F-KOK-535 // KOKUMI · IFI-K

Betaine Kokumi II-IV-A

Capsicum annuum

Pepper

Tier IIBetaine Kokumi

Target Trait

1.38×

fold-change

Glycine betaine CaSR interaction fold-change in Capsicum annuum

Technical Specifications

SKU

SS-F-KOK-535

Genomic Edits

4

GC Content

45.4%

Construct Length

2,877 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

138

Pathway Edits

  • BADH (betaine aldehyde dehydrogenase) overexpression for choline-to-betaine oxidation
  • CMOB (choline monooxygenase B) expression for betaine aldehyde intermediate generation
  • CMO (choline monooxygenase) upregulation for choline oxidation pathway activation
  • BCSA (betaine choline sodium antiporter) upregulation for cytosolic betaine accumulation
  • Betaine-GABA transporter-like protein expression for extracellular betaine release

Key Interventions

CMO/BADH two-step betaine biosynthesis from choline for CaSR allosteric modulator accumulationBCSA antiporter upregulation for cytosolic betaine pool expansion in Capsicum annuumBetaine transport expression for extracellular release and CaSR allosteric site accessCell-free in vitro transcription/translation delivery optimized for Capsicum annuum genome accessibilityTier-II construct validated with 4 precision edits for 1.38× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
138
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Pepper-derived betaine kokumi foundation for mild mouthfulness augmentation in pilot formulations

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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