IFI-K · Betaine Kokumi

Specimen SS-F-KOK-528 // KOKUMI · IFI-K

Betaine Kokumi I-IV-B

Capsicum annuum

Pepper

Tier IBetaine Kokumi

Target Trait

1.08×

fold-change

Glycine betaine CaSR interaction fold-change in Capsicum annuum

Technical Specifications

SKU

SS-F-KOK-528

Genomic Edits

4

GC Content

46%

Construct Length

2,727 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

108

Pathway Edits

  • BADH (betaine aldehyde dehydrogenase) overexpression for choline-to-betaine oxidation
  • CMOB (choline monooxygenase B) expression for betaine aldehyde intermediate generation
  • CMO (choline monooxygenase) upregulation for choline oxidation pathway activation
  • BCSA (betaine choline sodium antiporter) upregulation for cytosolic betaine accumulation
  • Betaine-GABA transporter-like protein expression for extracellular betaine release

Key Interventions

CMO/BADH two-step betaine biosynthesis from choline for CaSR allosteric modulator accumulationBCSA antiporter upregulation for cytosolic betaine pool expansion in Capsicum annuumBetaine transport expression for extracellular release and CaSR allosteric site accessCell-free in vitro transcription/translation delivery optimized for Capsicum annuum genome accessibilityTier-I construct validated with 4 precision edits for 1.08× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
108
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Research-grade betaine-CaSR kokumi modulator for allosteric interaction mechanism studies

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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