IFI-K · L-Carnosine Analog

Specimen SS-F-KOK-504 // KOKUMI · IFI-K

L-Carnosine Analog III-IV-B

Capsicum annuum

Pepper

Tier IIIL-Carnosine Analog

Target Trait

2.48×

fold-change

β-alanyl-L-histidine dipeptide kokumi fold-change in Capsicum annuum

Technical Specifications

SKU

SS-F-KOK-504

Genomic Edits

6

GC Content

48.9%

Construct Length

3,541 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

248

Pathway Edits

  • CNDP1 (carnosine dipeptidase 1) active-site remodeling for anabolic carnosine synthesis
  • CarsSyn (carnosine synthase) expression cassette for direct β-alanyl-His dipeptide production
  • ALT (alanine aminotransferase) expression for β-alanine precursor supply to CarsSyn
  • Ala-AT (alanine aminotransferase) rebalancing to redirect alanine flux toward β-alanine production
  • Carnosine permease upregulation for cytosolic dipeptide pool expansion
  • CNDP2 cytosolic dipeptidase silencing to prevent carnosine catabolism

Key Interventions

CNDP1/CarsSyn co-expression for anabolic carnosine analog dipeptide biosynthesis as CaSR kokumi ligandALT/Ala-AT β-alanine precursor supply engineering for CarsSyn substrate availabilityCNDP2 catabolism silencing to maintain carnosine analog pool for sustained CaSR engagementCell-free in vitro transcription/translation delivery optimized for Capsicum annuum genome accessibilityTier-III construct validated with 6 precision edits for 2.48× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
248
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial carnosine analog extract for savory snack and hot sauce manufacture

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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