IFI-K · Spermidine CaSR

Specimen SS-F-KOK-462 // KOKUMI · IFI-K

Spermidine CaSR III-PR-B

Allium sativum

Garlic

Tier IIISpermidine CaSR

Target Trait

2.11×

fold-change

Spermidine polyamine CaSR ligand fold-change in Allium sativum

Technical Specifications

SKU

SS-F-KOK-462

Genomic Edits

6

GC Content

47.6%

Construct Length

3,627 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-4000

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

211

Pathway Edits

  • SPDS (spermidine synthase) overexpression cassette for direct spermidine biosynthesis amplification
  • ADC (arginine decarboxylase) expression for agmatine-to-putrescine pathway activation
  • ODC (ornithine decarboxylase) activity enhancement for putrescine precursor supply
  • SAMDC (S-adenosylmethionine decarboxylase) overexpression for aminopropyl donor availability
  • PAO (polyamine oxidase) silencing to block spermidine retroconversion to putrescine
  • Spermidine vacuolar export transporter upregulation for cytosolic accumulation

Key Interventions

SPDS overexpression for direct spermidine biosynthesis as CaSR polyamine ligand in AlliumADC/ODC dual-pathway activation for high-flux putrescine supply to SPDS spermidine synthesisPAO retroconversion silencing to prevent spermidine catabolism and maximize steady-state poolProtoplast PEG-fusion delivery optimized for Allium sativum genome accessibilityTier-III construct validated with 6 precision edits for 2.11× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
211
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial spermidine-enriched garlic extract for savory snack and broth manufacture

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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