IFI-K · Spermidine CaSR

Specimen SS-F-KOK-453 // KOKUMI · IFI-K

Spermidine CaSR II-PR-A

Allium sativum

Garlic

Tier IISpermidine CaSR

Target Trait

1.52×

fold-change

Spermidine polyamine CaSR ligand fold-change in Allium sativum

Technical Specifications

SKU

SS-F-KOK-453

Genomic Edits

4

GC Content

44.6%

Construct Length

2,883 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-4000

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

152

Pathway Edits

  • SPDS (spermidine synthase) overexpression cassette for direct spermidine biosynthesis amplification
  • ADC (arginine decarboxylase) expression for agmatine-to-putrescine pathway activation
  • ODC (ornithine decarboxylase) activity enhancement for putrescine precursor supply
  • SAMDC (S-adenosylmethionine decarboxylase) overexpression for aminopropyl donor availability
  • PAO (polyamine oxidase) silencing to block spermidine retroconversion to putrescine
  • Spermidine vacuolar export transporter upregulation for cytosolic accumulation

Key Interventions

SPDS overexpression for direct spermidine biosynthesis as CaSR polyamine ligand in AlliumADC/ODC dual-pathway activation for high-flux putrescine supply to SPDS spermidine synthesisPAO retroconversion silencing to prevent spermidine catabolism and maximize steady-state poolProtoplast PEG-fusion delivery optimized for Allium sativum genome accessibilityTier-II construct validated with 4 precision edits for 1.52× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
152
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Garlic-derived polyamine kokumi foundation for mild mouthfulness augmentation in pilot formulations

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Kokumi · IFI-KAll Modalities