IFI-K · Spermidine CaSR

Specimen SS-F-KOK-441 // KOKUMI · IFI-K

Spermidine CaSR I-AG-A

Allium sativum

Garlic

Tier ISpermidine CaSR

Target Trait

1.1×

fold-change

Spermidine polyamine CaSR ligand fold-change in Allium sativum

Technical Specifications

SKU

SS-F-KOK-441

Genomic Edits

3

GC Content

44.2%

Construct Length

2,468 bp

Promoter System

Tissue-specific 2A11 / CaMV 35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

110

Pathway Edits

  • SPDS (spermidine synthase) overexpression cassette for direct spermidine biosynthesis amplification
  • ADC (arginine decarboxylase) expression for agmatine-to-putrescine pathway activation
  • ODC (ornithine decarboxylase) activity enhancement for putrescine precursor supply
  • SAMDC (S-adenosylmethionine decarboxylase) overexpression for aminopropyl donor availability
  • PAO (polyamine oxidase) silencing to block spermidine retroconversion to putrescine
  • Spermidine vacuolar export transporter upregulation for cytosolic accumulation

Key Interventions

SPDS overexpression for direct spermidine biosynthesis as CaSR polyamine ligand in AlliumADC/ODC dual-pathway activation for high-flux putrescine supply to SPDS spermidine synthesisPAO retroconversion silencing to prevent spermidine catabolism and maximize steady-state poolAgrobacterium-mediated T-DNA delivery optimized for Allium sativum genome accessibilityTier-I construct validated with 3 precision edits for 1.1× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
110
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Research-grade spermidine-CaSR kokumi reference for polyamine ligand mechanism studies

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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