IFI-K · GABA-CaSR

Specimen SS-F-KOK-420 // KOKUMI · IFI-K

GABA-CaSR III-BL-B

Capsicum annuum

Pepper

Tier IIIGABA-CaSR

Target Trait

2.27×

fold-change

GABA-mediated CaSR allosteric modulation fold-change in Capsicum annuum

Technical Specifications

SKU

SS-F-KOK-420

Genomic Edits

6

GC Content

46.5%

Construct Length

3,513 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle delivery

Host Strain

0.6µm Gold Particle

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

227

Pathway Edits

  • GAD1 (glutamate decarboxylase 1) overexpression cassette for high-flux GABA biosynthesis
  • GAD2 isoform co-expression for redundant GABA production under varied pH conditions
  • SSADH-RNAi (succinate semialdehyde dehydrogenase) silencing to block GABA catabolism
  • POP2 (pollen-pistil incompatibility 2) GABA transaminase attenuation via RNAi
  • GABA permease upregulation for cytosolic GABA pool accumulation and export
  • PYR/PYL ABA receptor modulation to relieve GAD1 inhibitory phosphorylation

Key Interventions

GAD1/GAD2 co-expression for redundant high-flux GABA biosynthesis from glutamate in CapsicumSSADH and POP2 dual silencing to prevent GABA catabolism and maximize steady-state poolGABA permease upregulation for cytosolic and extracellular CaSR allosteric modulator accumulationBiolistic particle delivery delivery optimized for Capsicum annuum genome accessibilityTier-III construct validated with 6 precision edits for 2.27× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
227
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial GABA-enriched pepper extract for sauce, condiment and hot sauce manufacture

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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