IFI-K · GABA-CaSR

Specimen SS-F-KOK-416 // KOKUMI · IFI-K

GABA-CaSR II-IV-B

Capsicum annuum

Pepper

Tier IIGABA-CaSR

Target Trait

1.78×

fold-change

GABA-mediated CaSR allosteric modulation fold-change in Capsicum annuum

Technical Specifications

SKU

SS-F-KOK-416

Genomic Edits

5

GC Content

47.3%

Construct Length

3,234 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

178

Pathway Edits

  • GAD1 (glutamate decarboxylase 1) overexpression cassette for high-flux GABA biosynthesis
  • GAD2 isoform co-expression for redundant GABA production under varied pH conditions
  • SSADH-RNAi (succinate semialdehyde dehydrogenase) silencing to block GABA catabolism
  • POP2 (pollen-pistil incompatibility 2) GABA transaminase attenuation via RNAi
  • GABA permease upregulation for cytosolic GABA pool accumulation and export
  • PYR/PYL ABA receptor modulation to relieve GAD1 inhibitory phosphorylation

Key Interventions

GAD1/GAD2 co-expression for redundant high-flux GABA biosynthesis from glutamate in CapsicumSSADH and POP2 dual silencing to prevent GABA catabolism and maximize steady-state poolGABA permease upregulation for cytosolic and extracellular CaSR allosteric modulator accumulationCell-free in vitro transcription/translation delivery optimized for Capsicum annuum genome accessibilityTier-II construct validated with 5 precision edits for 1.78× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
178
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Pepper-derived GABA kokumi foundation for mild mouthfulness enhancement in pilot formulations

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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