IFI-K · GABA-CaSR

Specimen SS-F-KOK-413 // KOKUMI · IFI-K

GABA-CaSR II-PR-A

Capsicum annuum

Pepper

Tier IIGABA-CaSR

Target Trait

1.65×

fold-change

GABA-mediated CaSR allosteric modulation fold-change in Capsicum annuum

Technical Specifications

SKU

SS-F-KOK-413

Genomic Edits

4

GC Content

44.6%

Construct Length

2,803 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-4000

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

165

Pathway Edits

  • GAD1 (glutamate decarboxylase 1) overexpression cassette for high-flux GABA biosynthesis
  • GAD2 isoform co-expression for redundant GABA production under varied pH conditions
  • SSADH-RNAi (succinate semialdehyde dehydrogenase) silencing to block GABA catabolism
  • POP2 (pollen-pistil incompatibility 2) GABA transaminase attenuation via RNAi
  • GABA permease upregulation for cytosolic GABA pool accumulation and export
  • PYR/PYL ABA receptor modulation to relieve GAD1 inhibitory phosphorylation

Key Interventions

GAD1/GAD2 co-expression for redundant high-flux GABA biosynthesis from glutamate in CapsicumSSADH and POP2 dual silencing to prevent GABA catabolism and maximize steady-state poolGABA permease upregulation for cytosolic and extracellular CaSR allosteric modulator accumulationProtoplast PEG-fusion delivery optimized for Capsicum annuum genome accessibilityTier-II construct validated with 4 precision edits for 1.65× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
165
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Pepper-derived GABA kokumi foundation for mild mouthfulness enhancement in pilot formulations

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Kokumi · IFI-KAll Modalities