IFI-K · Alliin Enhancement

Specimen SS-F-KOK-391 // KOKUMI · IFI-K

Alliin Enhancement IV-IV-A

Allium sativum

Garlic

Tier IVAlliin Enhancement

Target Trait

3.53×

fold-change

Alliin (S-allyl-L-cysteine sulfoxide) precursor fold-change vs baseline

Technical Specifications

SKU

SS-F-KOK-391

Genomic Edits

7

GC Content

44.8%

Construct Length

4,031 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

353

Pathway Edits

  • ACSO-high (high-activity alliin synthase) variant expression via strong constitutive promoter
  • FMO1 (flavin-containing monooxygenase 1) sulfoxidation activity enhancement for ACSO accumulation
  • CS-lyase modulation for cysteine-to-alliin precursor flux control
  • GCL supply-chain enhancement for cysteine intermediate availability
  • Vacuolar alliin sequestration channel optimization for storage and stability
  • CMK1 (cysteine monooxygenase kinase 1) regulatory phosphorylation site removal

Key Interventions

ACSO-high variant expression to amplify alliin biosynthesis as CaSR-active kokumi precursorFMO1 sulfoxidation enhancement for high-flux S-allyl-L-cysteine to alliin conversionCS-lyase balance tuning to partition cysteine sulfoxide flux toward alliin accumulationCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-IV construct validated with 7 precision edits for 3.53× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
353
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium garlic alliin isolate for restaurant and culinary specialty applications

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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