IFI-K · Alliin Enhancement

Specimen SS-F-KOK-361 // KOKUMI · IFI-K

Alliin Enhancement I-AG-A

Allium sativum

Garlic

Tier IAlliin Enhancement

Target Trait

1.5×

fold-change

Alliin (S-allyl-L-cysteine sulfoxide) precursor fold-change vs baseline

Technical Specifications

SKU

SS-F-KOK-361

Genomic Edits

3

GC Content

44.2%

Construct Length

2,508 bp

Promoter System

Tissue-specific 2A11 / CaMV 35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

150

Pathway Edits

  • ACSO-high (high-activity alliin synthase) variant expression via strong constitutive promoter
  • FMO1 (flavin-containing monooxygenase 1) sulfoxidation activity enhancement for ACSO accumulation
  • CS-lyase modulation for cysteine-to-alliin precursor flux control
  • GCL supply-chain enhancement for cysteine intermediate availability
  • Vacuolar alliin sequestration channel optimization for storage and stability
  • CMK1 (cysteine monooxygenase kinase 1) regulatory phosphorylation site removal

Key Interventions

ACSO-high variant expression to amplify alliin biosynthesis as CaSR-active kokumi precursorFMO1 sulfoxidation enhancement for high-flux S-allyl-L-cysteine to alliin conversionCS-lyase balance tuning to partition cysteine sulfoxide flux toward alliin accumulationAgrobacterium-mediated T-DNA delivery optimized for Allium sativum genome accessibilityTier-I construct validated with 3 precision edits for 1.5× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
150
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Research-grade alliin reference ingredient for precursor pathway screening and R&D

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Kokumi · IFI-KAll Modalities