IFI-K · S-Allyl-Cysteine

Specimen SS-F-KOK-359 // KOKUMI · IFI-K

S-Allyl-Cysteine V-IV-A

Allium sativum

Garlic

Tier VS-Allyl-Cysteine

Target Trait

4.5×

fold-change

SAC (S-allyl-L-cysteine) CaSR agonist fold-change vs baseline

Technical Specifications

SKU

SS-F-KOK-359

Genomic Edits

9

GC Content

45.3%

Construct Length

4,678 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

450

Pathway Edits

  • ACSO-lyase (alliin lyase) expression for S-allyl cysteine sulfoxide to SAC conversion
  • GCL upregulation for cysteine-precursor supply to S-allylation pathway
  • CS-lyase (cystathionine γ-lyase) activity enhancement for C-S bond formation
  • SAM-SAT (S-adenosylmethionine dependent allyl transferase) allyl group donor pathway
  • Allyl-CoA channel engineering for cytosolic S-allyl donor availability
  • CSE (cystathionine β-synthase-related enzyme) expression for cysteine intermediate
  • Cysteine sulfoxide permease upregulation for SAC extracellular release and bioavailability

Key Interventions

ACSO-lyase expression to establish SAC biosynthesis as potent CaSR agonist in Allium sativumGCL cysteine precursor supply and CS-lyase C-S bond formation for allyl-cysteine scaffoldSAM-SAT allyl activation pathway for high-flux S-allyl group incorporation into cysteine acceptorCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-V construct validated with 9 precision edits for 4.5× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
450
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Ultra-premium SAC ingredient for luxury kokumi product differentiation

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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