IFI-K · Gamma-Glu-Cys

Specimen SS-F-KOK-304 // KOKUMI · IFI-K

Gamma-Glu-Cys III-IV-B

Allium sativum

Garlic

Tier IIIGamma-Glu-Cys

Target Trait

2.92×

fold-change

γ-Glu-Cys dipeptide fold-change vs baseline in Allium sativum

Technical Specifications

SKU

SS-F-KOK-304

Genomic Edits

7

GC Content

48.9%

Construct Length

3,921 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

292

Pathway Edits

  • GCL-high (high-activity GCL variant) overexpression via strong constitutive promoter
  • Gly-I-RNAi (glyoxalase I) silencing to prevent competitive GSH completion and retain γ-Glu-Cys
  • gamma-ECS (γ-glutamylcysteine synthetase) activity optimization for dipeptide accumulation
  • ACS1 (1-aminocyclopropane-1-carboxylate synthase 1) carbon flux rerouting to cysteine precursors
  • Cysteine transporter upregulation for cytosolic cysteine pool expansion
  • GGT4 retrograde catabolism attenuation to stabilize accumulated γ-Glu-Cys pool

Key Interventions

GCL-high amplification for γ-Glu-Cys dipeptide as direct CaSR kokumi ligand without GSH completionGly-I-RNAi silencing to prevent competing reactions and maximize γ-Glu-Cys pool for CaSR engagementgamma-ECS intermediate channeling to favor dipeptide accumulation over tripeptide completionCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-III construct validated with 7 precision edits for 2.92× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
292
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial γ-Glu-Cys concentrate for sauce and seasoning kokumi enhancement

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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