IFI-K · Gamma-Glu-Cys

Specimen SS-F-KOK-294 // KOKUMI · IFI-K

Gamma-Glu-Cys II-PR-B

Allium sativum

Garlic

Tier IIGamma-Glu-Cys

Target Trait

2.21×

fold-change

γ-Glu-Cys dipeptide fold-change vs baseline in Allium sativum

Technical Specifications

SKU

SS-F-KOK-294

Genomic Edits

5

GC Content

46.8%

Construct Length

3,160 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-4000

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

221

Pathway Edits

  • GCL-high (high-activity GCL variant) overexpression via strong constitutive promoter
  • Gly-I-RNAi (glyoxalase I) silencing to prevent competitive GSH completion and retain γ-Glu-Cys
  • gamma-ECS (γ-glutamylcysteine synthetase) activity optimization for dipeptide accumulation
  • ACS1 (1-aminocyclopropane-1-carboxylate synthase 1) carbon flux rerouting to cysteine precursors
  • Cysteine transporter upregulation for cytosolic cysteine pool expansion
  • GGT4 retrograde catabolism attenuation to stabilize accumulated γ-Glu-Cys pool

Key Interventions

GCL-high amplification for γ-Glu-Cys dipeptide as direct CaSR kokumi ligand without GSH completionGly-I-RNAi silencing to prevent competing reactions and maximize γ-Glu-Cys pool for CaSR engagementgamma-ECS intermediate channeling to favor dipeptide accumulation over tripeptide completionProtoplast PEG-fusion delivery optimized for Allium sativum genome accessibilityTier-II construct validated with 5 precision edits for 2.21× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
221
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Garlic-derived kokumi foundation for pilot broth and stock fortification

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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