IFI-K · Gamma-Glu-Val-Gly

Specimen SS-F-KOK-256 // KOKUMI · IFI-K

Gamma-Glu-Val-Gly II-IV-B

Saccharomyces cerevisiae

Yeast

Tier IIGamma-Glu-Val-Gly

Target Trait

2.08×

fold-change

γ-Glu-Val-Gly tripeptide fold-change vs wild-type yeast titre

Technical Specifications

SKU

SS-F-KOK-256

Genomic Edits

5

GC Content

47.3%

Construct Length

3,114 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

208

Pathway Edits

  • GGT1 (γ-glutamyl transpeptidase 1) Val-Gly specificity maturation via active-site remodeling
  • GGT4 isoform expression cassette for parallel γ-glutamyl transfer pathway activation
  • ALT2 (alanine aminotransferase 2) competitive glutamate sink removal for GGT substrate enrichment
  • Vacuolar V-type protease expression for dipeptide scaffold liberation from storage protein
  • γ-Glu-Val-Gly tripeptide vacuolar export permease upregulation for cytosolic accumulation
  • GDH1 (glutamate dehydrogenase 1) overexpression for GGT donor substrate pool expansion
  • GAD1 partial silencing to redirect glutamate from GABA shunt toward GGT reaction

Key Interventions

GGT1/GGT4 dual-isoform expression for high-flux γ-Glu-Val-Gly tripeptide biosynthesis via parallel routesALT2 competitor silencing to redirect free glutamate to γ-glutamyl transfer reactions at GGTVacuolar export permease upregulation to accumulate extracellular γ-Glu-Val-Gly kokumi poolCell-free in vitro transcription/translation delivery optimized for Saccharomyces cerevisiae genome accessibilityTier-II construct validated with 5 precision edits for 2.08× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
208
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Fermented condiment kokumi foundation for soy sauce and miso analog applications

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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