IFI-K · Gamma-Glu-Val-Gly
Specimen SS-F-KOK-245 // KOKUMI · IFI-K
Gamma-Glu-Val-Gly I-PR-A
Saccharomyces cerevisiae
Yeast
Tier IGamma-Glu-Val-Gly
Target Trait
1.3×
fold-change
γ-Glu-Val-Gly tripeptide fold-change vs wild-type yeast titre
Technical Specifications
SKU
SS-F-KOK-245
Genomic Edits
3
GC Content
43.9%
Construct Length
2,536 bp
Promoter System
Ubiquitin / T7
Expression System
Protoplast PEG-fusion
Host Strain
PEG-4000
Receptor Target
CaSR (calcium-sensing receptor)
IFI Score
130
Pathway Edits
- GGT1 (γ-glutamyl transpeptidase 1) Val-Gly specificity maturation via active-site remodeling
- GGT4 isoform expression cassette for parallel γ-glutamyl transfer pathway activation
- ALT2 (alanine aminotransferase 2) competitive glutamate sink removal for GGT substrate enrichment
- Vacuolar V-type protease expression for dipeptide scaffold liberation from storage protein
- γ-Glu-Val-Gly tripeptide vacuolar export permease upregulation for cytosolic accumulation
- GDH1 (glutamate dehydrogenase 1) overexpression for GGT donor substrate pool expansion
- GAD1 partial silencing to redirect glutamate from GABA shunt toward GGT reaction
Key Interventions
GGT1/GGT4 dual-isoform expression for high-flux γ-Glu-Val-Gly tripeptide biosynthesis via parallel routesALT2 competitor silencing to redirect free glutamate to γ-glutamyl transfer reactions at GGTVacuolar export permease upregulation to accumulate extracellular γ-Glu-Val-Gly kokumi poolProtoplast PEG-fusion delivery optimized for Saccharomyces cerevisiae genome accessibilityTier-I construct validated with 3 precision edits for 1.3× fold-change
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K130
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
Yeast-platform kokumi screening ingredient for γ-Glu-Val-Gly library validation and R&D
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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