IFI-K · Glutathione Enhancement
Specimen SS-F-KOK-232 // KOKUMI · IFI-K
Glutathione Enhancement IV-IV-B
Allium sativum
Garlic
Tier IVGlutathione Enhancement
Target Trait
4.05×
fold-change
GSH biosynthesis fold-change via GCL/GS overexpression
Technical Specifications
SKU
SS-F-KOK-232
Genomic Edits
9
GC Content
46.2%
Construct Length
4,688 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free in vitro transcription/translation
Host Strain
T7 RNA Polymerase
Receptor Target
CaSR (calcium-sensing receptor)
IFI Score
405
Pathway Edits
- GCL (γ-glutamylcysteine synthetase) catalytic subunit overexpression cassette insertion
- GS (glutathione synthetase) promoter swap to constitutive UBI1 for persistent expression
- GSR (glutathione reductase) copy-number amplification for sustained NADPH-dependent recycling
- gamma-ECS intermediate channel optimization to prevent rate-limiting precursor accumulation
- Gly-I (glyoxalase I) competitive cytosolic drain silencing via targeted RNAi
- Vacuolar GSH export transporter upregulation for cytosolic pool expansion
- GGT1 retrograde hydrolysis attenuation to prevent GSH catabolism by transpeptidase
Key Interventions
GCL/GS co-overexpression for direct GSH pool amplification targeting CaSR γ-glutamyl substrateGSR recycling loop enhancement for sustained reduced glutathione availability at CaSR binding sitesgamma-ECS intermediate accumulation to load CaSR γ-glutamyl peptide kokumi substrate poolCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-IV construct validated with 9 precision edits for 4.05× fold-change
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K405
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
Premium garlic-derived kokumi isolate for restaurant and specialty food-service applications
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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