Glutathione Enhancement IV-BL-B

Allium sativum

Garlic

Tier IVGlutathione Enhancement

Target Trait

4.05×

fold-change

GSH biosynthesis fold-change via GCL/GS overexpression

Technical Specifications

SKU

SS-F-KOK-228

Genomic Edits

GC Content

47%

Construct Length

4,620 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle delivery

Host Strain

0.6µm Gold Particle

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

405

Pathway Edits

GCL (γ-glutamylcysteine synthetase) catalytic subunit overexpression cassette insertion
GS (glutathione synthetase) promoter swap to constitutive UBI1 for persistent expression
GSR (glutathione reductase) copy-number amplification for sustained NADPH-dependent recycling
gamma-ECS intermediate channel optimization to prevent rate-limiting precursor accumulation
Gly-I (glyoxalase I) competitive cytosolic drain silencing via targeted RNAi
Vacuolar GSH export transporter upregulation for cytosolic pool expansion
GGT1 retrograde hydrolysis attenuation to prevent GSH catabolism by transpeptidase

Key Interventions

GCL/GS co-overexpression for direct GSH pool amplification targeting CaSR γ-glutamyl substrateGSR recycling loop enhancement for sustained reduced glutathione availability at CaSR binding sitesgamma-ECS intermediate accumulation to load CaSR γ-glutamyl peptide kokumi substrate poolBiolistic particle delivery delivery optimized for Allium sativum genome accessibilityTier-IV construct validated with 9 precision edits for 4.05× fold-change

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

405

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Premium garlic-derived kokumi isolate for restaurant and specialty food-service applications

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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