Glutathione Enhancement III-AG-A

Allium sativum

Garlic

Tier IIIGlutathione Enhancement

Target Trait

3×

fold-change

GSH biosynthesis fold-change via GCL/GS overexpression

Technical Specifications

SKU

SS-F-KOK-217

Genomic Edits

GC Content

43.7%

Construct Length

4,042 bp

Promoter System

Tissue-specific 2A11 / CaMV 35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

300

Pathway Edits

GCL (γ-glutamylcysteine synthetase) catalytic subunit overexpression cassette insertion
GS (glutathione synthetase) promoter swap to constitutive UBI1 for persistent expression
GSR (glutathione reductase) copy-number amplification for sustained NADPH-dependent recycling
gamma-ECS intermediate channel optimization to prevent rate-limiting precursor accumulation
Gly-I (glyoxalase I) competitive cytosolic drain silencing via targeted RNAi
Vacuolar GSH export transporter upregulation for cytosolic pool expansion
GGT1 retrograde hydrolysis attenuation to prevent GSH catabolism by transpeptidase

Key Interventions

GCL/GS co-overexpression for direct GSH pool amplification targeting CaSR γ-glutamyl substrateGSR recycling loop enhancement for sustained reduced glutathione availability at CaSR binding sitesgamma-ECS intermediate accumulation to load CaSR γ-glutamyl peptide kokumi substrate poolAgrobacterium-mediated T-DNA delivery optimized for Allium sativum genome accessibilityTier-III construct validated with 7 precision edits for 3× fold-change

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

300

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Commercial-grade garlic kokumi extract for sauce, condiment and seasoning manufacture

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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